Submitted to: National Center for Biotechnology Information (NCBI)
Publication Type: Database / Dataset
Publication Acceptance Date: 4/23/2025
Publication Date: 4/28/2025
Citation: Temeyer, K.B., Schlechte, K.G., Olafson, P.U., Tidwell, J.P. 2025. Acetylcholinesterase 1 of the North American cattle tick, Rhipicephalus (Boophilus) annulatus (Say). National Center for Biotechnology Information (NCBI). PV554210.

Interpretive Summary: The cattle fever ticks Rhipicephalus microplus and Rhipicephalus annulatus transmit pathogens responsible for the serious cattle diseases bovine babesiosis and anaplasmosis. Although these ticks were eradicated from the United States, they and the pathogens they transmit still are endemic in Mexico and other countries, and remain a serious risk to the U.S. Cattle industry. Imported cattle are inspected and treated with acaricide to keep these ticks and pathogens from re-entry into the United States, and outbreak infestations within the united States are eradicated under the Cattle Fever Tick Eradication Program administered through the Veterinary Services Division of the Animal and Plant Inspection Service of the U.S. Department of Agriculture in cooperation with Animal Health Agencies of various states. The acaricide of first choice for treatment of imported cattle and eradication of outbreak infestations is the organophosphate, coumaphos, which kills ticks by inactivating acetylcholinesterase, an enzyme vital to function of the tick central nervous system. New research reports construction and biochemical properties of an acaricide-resistant form of the acetylcholinesterase 1 of Rhipicephalus annulatus, a species of cattle fever tick. This research adds to the knowledge base necessary to enable further research to elucidate potential functional differences and increase understanding of tick physiology and genetics potentially helping with the development of improved tick control technologies.

Technical Abstract: Three mRNA sequences encoding acetylcholinesterase 1 of the cattle fever tick Rhipicephalus annulatus strain Vega differing at their 5'-ends and encoding different probable transcriptional start sites were previously submitted to the GenBank database of the National Center for Biotechnology Information at https://www.ncbi.nlm.nih.gov/. GenBank accession numbers for the submissions were assigned as PV236116, PV236117, PV236118. The nucleotide and amino acid sequences of a new construct of these RannAChE1s that is highly resistant to organophosphate and carbamate acaricides containing the amino acid substitution orthologous to the G119S mutation in mosquito acetylcholinesterase was submitted to the GenBank database of the National Center for Biotechnology Information at https://www.ncbi.nlm.nih.gov/. The GenBank accession number for the G119S orthologous submission was assigned as PV554210.